滚球体育

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浅谈薄层色谱
点击次数:1687 发布时间:2018-01-09
薄层色谱法(thin layer chromatography,TLC)是平面色谱法中应用zui广泛的方法之一,具有分离速度快、灵敏度高、显色方便、操作简便等优点。按分离机理可分为吸附、分配、离子交换、排阻色谱等,应用zui广泛的为吸附薄层色谱法。以下就简单的介绍吸附薄层色谱法。

RONGJIJIXING


 

JIESHAOXIFUBOCENGSEPUQIAN,YINGSHUZHICHANGYONGRONGJIJIXINGSHUNXUBIAO,BIANYUGENGHAODIYINGYONGBOCENGSEPU。CHANGYONGRONGJIJIXINGSHUNXUWEI:SHIYOUMI<HUANJIWAN<SILVHUATAN<SANLVYIXI<BEN<JIABEN<ERLVJIAWAN<YIMI<LVFANG<YISUANYIZHI<BINGTONG<ZHENGDINGCHUN<YICHUN<JIACHUN<SHUI<YISUAN。CIWAI,CHANGJIANHUAHEWUJIXINGSHUNXUWEI:WANTING<XITING<MI<XIAOJIHUAHEWU<ERJIAAN<ZHILEI<TONGLEI<QUANLEI<LIUCHUN<ANLEI<XIANANLEI<CHUNLEI<FENLEI<SUOSUANLEI。


 

 

ZHANKAIJIXUANZE


 

ZHANKAIJIDEJIXINGYUEQIANG,QIXITUONENGLIYUEQIANG,HUAHEWUDERfZHIYUEDA;FANZHENGZHANKAIJIJIXINGYUEXIAO,XITUONENGLIRUO,XIANGYINGHUAHEWUDERfZHIYUEXIAO,YIBANYAOQIUXUANQUHESHIDEZHANKAIJIXITONGSHIHUAHEWUDERfZHIKONGZHIZAI0.2-0.8YINEI。ZHANKAIJIXUANZEKEYIYONGDIANDISHIYANFA,JIANGYAOBEIFENLIDEWUZHIRONGYEDIANZAIBOCENGBANSHANG,HUIGAN,YONGMAOXIGUANFENBIEXIMANBUTONGZHANKAIJIDIANDAOYANGPINDIANDEZHONGXIN,JIEMAOXIGUANZUOYONG,ZHANKAIJICONGYUANXINXIANGWAIKUOZHAN,CHUXIANBUTONGDEYUANXINSEPU,XUANQUzuiHESHIDEZHANKAIJI。RUXIATU,BENWEIzuiHESHIDEZHANKAIJI。



 


 

为了提高分离效果,经常需要采用混合溶剂组成流动相,调整比例及更换展开剂组成来调整流动相的极性、互溶性、酸碱性和黏度,达到zui佳的分离目的。混合展开剂中,弱极性溶剂系统一般由石油醚、苯、环己烷等组成,然后选择性加入乙酸乙酯、甲醇、乙醇等;中等极性溶剂系统一般由三氯甲烷和水组成,用乙酸乙酯、甲醇、乙醇来调节,适合于蒽醌类或皂苷类成分分离;大极性溶剂系统一般由正丁醇和水组成,用乙酸乙酯、甲醇、乙醇来调,适合于极性较大的有机碱类化合物的分离。

BOCENGBANDELEIBIE


 

CHANGJIANDEBOCENGBANXINGHAOJIDAIBIAOHANYIRUXIABIAO:


 


 


 

DIANYANG


 

滚球体育DIANYANGXINGZHUANGYIBANWEIYUANXINGDIAN,DIANYANGJIXIANJUDIBIAN1.0-1.5cm,DIANYANGZHIJINGWEI2-3mm,BANYUANDIANZHIJINGYUEWEI1mm,JINLIANGBIMIANDUOCIDIANYANG。DIANJIANJULIGENJUBANDIANKUOSANQINGKUANGERDING,YIBANBOCENGWEI1-2cm,BOCENGWEI0.5cm。YONGSHOUGONGDIANYANGSHI,YINGGAIZHUYIWUSUNSHIBOCENGBIAOMING,JIYONGZUICHUIBOCENGBAN。


 


 

ZHANKAI


 

滚球体育DIANYANGHOUDEBOCENGBAN,ZHIYUMIBIDECENGXIGANGZHONGZHANKAI。ZHANKAIJIJINRUBOCENGBANXIADUANGAODUBUCHAOGUO0.5cm,BAOZHENGDIANYANGCHUBUJIECHUZHANKAIJI。

DUIYUMOUXIEFUZAHUONANFENLIDECHENGFEN,KECAIYONGSHUANGXIANGZHANKAIFAHUOZHEERCIZHANKAIFA,SHUANGXIANGZHANKAIJIZAIFANGXINGDEBOCENGBANDEXIANGLINLIANGBIANFENBIEHUAYITIAOZHIXIAN,XIANGJIAOYUYIDIANWEIYUANDIAN,JIANGYANGPINRONGYEDIANYUCIDIANSHANG,XIANYONGYIZHONGZHANKAIJIYANZHEYIGEFANGXIANGZHANKAI,CHUIGANZHANKAIJI,DIAOZHUANBOCENGBAN,ZAIYONGLINGYIZHONGZHANKAIJIJINXINGDIERCIZHANKAI,DUIFUZANANFENLIDEYANGPINYOUJIAOHAODEFENLIXIAOGUO,RUTU。


 


 

滚球体育CIWAI,ZHANKAIHOUDEBANKENENGHUICHUXIANBIANYUANXIAOYING。BIANYUANXIAOYINGSHIZHIDIANYUTONGYIBOCENGDETONGYIWUZHI,ZHANKAIHOUBOCENGBANBIANYUANCHUBANDIANDERfZHIYUBANZHONGJIANDEBANDIANRfZHIYOUSUOBUTONG,CHENGXIANAOXING,RUXIATU。


 


 


 

BIANYUANXIAOYINGDUOCHUXIANZAIHUNHERONGJIZHANKAIJIXITONGZHONG,RUGUOBOCENGBANJIAODA,ZHANKAISHIJIANZHANG,ZHANKAIJIHUIFADESUDUBUTONG,SHANGXINGZHANKAISHI,YOUYUBEIXIFUJIXIFUJIAORUODERUOJIXINGHUOYIHUIFADERONGJI,ZAIBOCENGBANBIANYUANYIHUIFA,SHIBOCENGBANBIANYUANBUFENRONGJIJIXINGBIZHONGJIANDA,BIANYUANBANDIANDERfZHIDAYUZHONGJIANQUYUDE;LINGYIFANGMIAN,BOCENGBANBEIMIANZHENGQIXIBO,DAOZHIBANBIANYUANCHUZHENGQIXIANGBEIMIANYIDONG,ZHISHIBIANYUANCHUZHENGFADIAODERONGJIYOURONGJIZHUCUNQIDEDAOBUCHONG,BANBIANYUANYUZHONGXINXIANGBI,YOUGENGDUODEZHANKAIJIYANBIANYUANYIDONG,YINCIBIANYUANDEBANDIANRfZHIGAOYUZHONGJIANQUYU。


 

WEILEJIANSHAOBIANYUANXIAOYING,KECAIQUYIXIABANFA:

  1. SHIYONGSHUANGCAODECENGXIGANG,YICAOZHONGFANGBOCENGBAN,LINGYICAOJIARUZHANKAIJIDAIRONGJIZHENGQIDADAOBAOHEHOUZAIZHANKAI;

  2. ZAICENGXIGANGNEIBITIESHANGJINSHIZHANKAIJIDELVZHITIAO;

  3. SHIYONGYUZHIBAN;

  4. SHIYONGJIAOXIAOTIJIDEHESHIDECENGXIGANG;

  5. 滚球体育JIANGBANDIANJINLIANGDIANZAIKAOZHONGJIANQUYU;

  6. CAIYONGXIABOCENGBANDAITIKUANBOCENGBAN;

  7. XUANZESHIYIDEDANYIZHANKAIJIDAITIHUNHEZHANKAIJI;

  8. 滚球体育CAIYONGGONGFEIRONGJIDAITIYIBANHUNHERONGJI。


 


 

BANDIANDEJIANSHI


 

色谱斑点的检识包括光学检出法试剂显色法

滚球体育GUANGXUEJIANCHUFAFENSANZHONGQINGKUANG:

  1. HUAHEWUBENSHENYOUSE,ZAIRIGUANGXIAKEZHIJIEGUANCHA;

  2. 滚球体育HUAHEWUZAIKEJIANGUANGXIAMEIYOUYANSE,DANKEXISHOUZIWAIGUANGBINGFASHEGENGZHANGBOZHANGDEGUANGERCHENGXIANBUTONGYANSEDEYINGGUANGBANDIAN;

  3. ZAIKEJIANZIWAIGUANGXIADOUBUXIANSE,YEMEIYOUHESHIXIANSEFANGFADE,KEYIYONGYINGGUANGBOCENGBAN,HUAHEWUZAIZIWAIDENGXIAKEZAIYINGGUANGBEIJINGXIAXIANSHIANBAN。ZHEISHIYINWEIZHEIXIEHUAHEWUJIANRUOLEXIFUJIZHONGYINGGUANGWUZHIDEZIWAIXISHOUQIANGDU,YINQIYINGGUANGCUMIE。


 

SHIJIXIANSEFABAOKUOYIXIASANZHONG:

  1. 滚球体育PENWUXIANSE,JIANGXIANSEJIPENSAYUBOCENGBAN(YINGBAN)SHANG,GENJUXIANSEYUANLIBUTONG,KEZHIJIEXIANSEHUOJIAREXIANSE。

  2. JINZIXIANSE。

  3. ZHENGQIXIANSE:RUDIANZHENGQI,HUIFAXINGDESUAN、JIAN,RUYANSUAN、XIAOSUAN、NONGANSHUI、YIERANDENG。


     


 


 

YICHANGBANDIANDEDUICE


 

7.1 拖尾  

TUOWEIZAIBOCENGZHONGJIAOCHANGJIAN。YINQITUOWEIDEYUANYINYOUYIXIAJIDIAN:1.DIANYANGGUOLIANG,YOUYUXIFUJIFUZAIYANGPINNENGLIYOUXIAN,CHAOZAIDEYANGPINBEIPAOZAIHOUMIAN,XINGCHENGTUOWEI。2.ZHONGFUDIANYANG,ZHONGFUDIANYANGSHIMEICIZHONGXINWEIZHONGHE,SHIDIANYANGDIANCHENGTUOYUANXING,XINGCHENGTUOWEI。3.GUIJIAOSIXIFUDADEHUAHEWUYIYIXINGCHENGTUOWEI,RUDUOQIANGJIHUAHEWU,YOUJISUAN,TANGGANDENG。GENJUSHANGSHUYUANYIN,NONGDEYANGPINKEYIXISHIZHIHOUZAIDIANYANG;ZHONGFUDIANYANGYANGPINKONGZHISHOUBUFADOUHUOZHEMEICIDIANYANGDOUKONGZHIDIANZAITONGYIWEIZHI;JIARUSUANJIANDENGGAILIANGJILAIJIANSHAOTUOWEIXIANXIANG。


 

7.2 念珠状斑点  

YOUYUHUAHEWUBANDIANZHIJIANJULIXIAO,XIANGHULIANJIECHENGNIANZHUZHUANG。YINQIYUANYINYOU:1.YANGPINCHENGFENFUZAGUODUO,ZAIDUANDEBOCENGBANSHANGDABUDAOFENLIXIAOGUO,BICIZHONGDIE。2.ZHONGFUDIANYANGSHI,ZHONGXINBUZHONGHE,XINGCHENGFUBAN。CAIQUCUOSHI:SHIDANGZENGJIABOCENGBANDEZHANGDUHUOCAIYONGERCISHUANGXIANGZHANKAI;ZHONGFUDIANYANGSHI,MEICIDIANYANGZHONGXINYINGZHONGHE。


 

7.3 Rf值不稳定滚球体育  

ZHONGFUCAOZUOSHITONGYIYANGPINRfZHIBUWENDING,SHIGAOSHIDI。RUXIAQINGKUANGDAOZHICIXIANXIANG:1.CENGXIWENDINGBUWENDING,TEBIESHISHIYONGHUNHEZHANKAIXITONGSHI,WENDUBUWENDINGDAOZHIZHANKAIJIBILIBIANHUA;2.RENWEICAOZUOBUDANG,XIQUHUOJIARUZHANKAIJISHIBUXUNSU,HUOJIARUZHANKAIJISHIWEIJISHIJIANGCENGXIGANGGAISHANGMIFENG;3.BOCENGBANHOUBOBUJUNYUN;4.RONGJIZHILIANGBUTONG。DUICE:RUGUOYONGDIANCHUIFENGCHUIGANCENGXIGANGSHIYIDINGDAICENGXIGANGLENGQUEZHISHIWENZAIZHANKAI;YANGCHENGZHENGQUEDESHIYANCAOZUOXIGUAN;XUANQUHOUBOJUNYIDEBOCENGBAN;XUANZEZHILIANGHAODERONGJI。

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